diff --git a/keyboards/keebio/nyquist/rev4/config.h b/keyboards/keebio/nyquist/rev4/config.h
index e1e2d994583..0a0f7881281 100644
--- a/keyboards/keebio/nyquist/rev4/config.h
+++ b/keyboards/keebio/nyquist/rev4/config.h
@@ -12,7 +12,3 @@
 
 #define RP2040_BOOTLOADER_DOUBLE_TAP_RESET
 #define RP2040_BOOTLOADER_DOUBLE_TAP_RESET_TIMEOUT 1000U
-
-#define I2C_DRIVER I2CD0
-#define I2C0_SDA_PIN GP4
-#define I2C0_SCL_PIN GP5
diff --git a/keyboards/keebio/nyquist/rev5/config.h b/keyboards/keebio/nyquist/rev5/config.h
index 8a88c21c891..c4bbd6c5b20 100644
--- a/keyboards/keebio/nyquist/rev5/config.h
+++ b/keyboards/keebio/nyquist/rev5/config.h
@@ -14,7 +14,3 @@
 
 #define RP2040_BOOTLOADER_DOUBLE_TAP_RESET
 #define RP2040_BOOTLOADER_DOUBLE_TAP_RESET_TIMEOUT 1000U
-
-#define I2C_DRIVER I2CD0
-#define I2C0_SDA_PIN GP4
-#define I2C0_SCL_PIN GP5